Monoclonal antibodies to rabbit sperm autoantigens. II. Inhibition of human sperm penetration of zona-free hamster eggs
نویسندگان
چکیده
منابع مشابه
Monoclonal antibodies to human sperm antigens--II.
As part of our continuous effort to elucidate the biochemical and immunological nature of human sperm surface antigens, monoclonal antibodies to human spermatozoa were generated by improved hybridoma techniques. Following immunizations with the membrane fraction of human spermatozoa and cell fusions, hybrid cells were cultured in a semi-solid HAT-selection medium to maximize the number of monoc...
متن کاملZona-free hamster ovum penetration by human spermatozoa: influence of various sperm factors.
The present paper evaluates the influence of various technical factors when preparing human spermatozoa to be tested for their ability to penetrate zona-free hamster ova. Higher in vitro fertilization rates were obtained after sperm selection by "swim-up" migration, 4-h incubation, sperm dilution to a concentration of 2.5 X 10(6) spermatozoa/ml, and observation of the oocytes 8 h after insemina...
متن کاملPreliminary study of buffalo sperm penetration into zona-free hamster eggs after treatment with calcium ionophore A23187.
A previous study (Takahashi & Hanada, 1984) reported the penetration of zona-free hamster eggs by ejaculated bull spermatozoa after treatment with calcium ionophore A23187 (Ca-IA) in the presence of caffeine. Based on this study, successful IVF was accomplished not only in cattle (Hanada, 1986b) but also in goats and sheep (Hanada, 1986a) using spermatozoa treated with Ca-IA. In the present stu...
متن کاملMonoclonal antibodies to human sperm antigens.
To elucidate the molecular nature of human sperm autoantigens, attempts were made to raise monoclonal antibodies against these antigens, by hybridoma techniques. After successive immunizations with the particulate fractions of human sperm extract in BALB/c mice, the spleen cells were fused with P3-X63-Ag8 myeloma cells. Several clones and their subclones were obtained and shown by microplate ra...
متن کاملInhibition of goat sperm-zona binding by monoclonal antibodies to a glycoprotein family (ZP4) of porcine zona pellucida
The zona pellucida (ZP) in a majority of species consists of three antigenic families with wide inter species cross reactivity. The glocoproteins of heat solubilized goat zona pellucida (gZP) were isolated into three families by high performance liquid chromatography on a gel ®ltration column. Their molecular identities were 180, 95 and 75 kDa for gZP1, gZP2 and gZP3 respectively. However under...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1984
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod30.3.731